不稳定铁离子(Fe2+)荧光检测试剂—— FerroFarRed™和FerroOrange

来源: 欣博盛生物科技有限公司   2019-5-31   访问量:436评论(0)

FerroFarRed(也称为SiRhoNox-1ER-SiRhoNox)是一种荧光探针,专门用于检测不稳定的二价铁离子(Fe2+)。该探针仅选择性地与其他金属离子Fe 2+反应,并且不可逆地变成一种远红荧光物质。它主要定位于内质网(ER)内。它也可以与配备红色激光的流式细胞仪一起使用。



FerroOrange是一种专门用于检测不稳定的二价铁离子(Fe2+橙色荧光探针。在三价铁离子(Fe3+)或除铁以外的其他二价金属离子存在情况下,荧光强度不会增加。它也不会铁蛋白其他物质中的络合发生反应。FerroOrange适用于活细胞成像,因为它具有高度的细胞渗透性和低细胞毒性。

产品信息

产品货号 产品名称 规格 靶标 细胞渗透性 反应 Absmax(nm) FLmax(nm)
GC903-01 FerroFarRed 50 nmol x 5 Fe2+ 不可逆 646 662

产品货号 产品名称 规格 靶标 细胞渗透性 反应 Absmax(nm) Emmax(nm)
GC904-01 FerroOrange 35 nmol x 5 Fe2+ 不可逆 542 572
GC904-02 35 nmol x 1


吸收光谱和荧光光谱

Absorption spectrum (left image) and fluorescence spectrum when excited at 630 nm (right image) of FerroFarRed. Reaction with Fe2+ significantly increases the fluorescence intensity to a maximum of 662 nm.

FerroOrange absorption spectrum (left) and fluorescence spectrum change (right)。 Due to reaction with Fe2+, the fluorescence intensity increases markedly maximizing at 572 nm wavelength。


活细胞成像实验

HeLa cells cultured for 30 minutes in serum-free medium containing 100 μM Fe(SO4)2(NH4)2 (right) and HeLa cells without loading Fe2+ (left). The cells were washed with HBSS to remove extracellular Fe2+ ions before reacting with 5 µM FerroFarRed at 37oC for 1 hour. The cells were observed under a fluorescence microscope using a 590-650 nm excitation filter and a 660-740 nm fluorescence filter. The magenta pseudo color shows the fluorescent image of FerroFarRed. Grayscale images of DIC and magenta pseudo color were overload.

Live cell imaging of HepG2 cells by FerroOrange without loading Fe2+ (left) and with loading Fe2+ (right). The image was taken after 1 hour culture at 37˚C 5% CO2 with HBSS containing 1 µM FerroOrange. A significant increase of fluorescence intensity can be seen on the image with Fe2+ (right) comparing to the image without Fe2+ (left). To load Fe2+, HepG2 cells were cultured for 30 minutes in HBSS containing 100 µM of Fe(SO4)2(NH4)2, and then washed with HBSS to remove extracellular Fe2+. The orange pseudo color shows the fluorescence image of FerroOrange. These cells were observed under a filter set with excitation wavelength 530-560 nm and fluorescence wavelength 570-650 nm.

有关分析方法和应用实例,请参阅应用说明和文献

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