Genes :北京林业大学丨盐胁迫下钾转运体LrKUP8抑制K+外排

来源: 旭月(北京)科技有限公司   2019-12-16   访问量:346评论(0)

NMT是基因功能的活体检测技术,已被103位诺贝尔奖得主所在单位,及北大、清华、中科院使用。

期刊:Genes

主题:盐胁迫下钾转运体LrKUP8抑制K+外排

标题:Potassium Transporter LrKUP8 Is Essential for K+Preservation in Lycium ruthenicum, A Salt-Resistant Desert Shrub

影响因子:3.331

检测指标:K+流速

检测样品:黒枸杞愈伤组织

K+流实验处理方法:

有/无300 mM NaCl培养12h

K+流实验测试液成份:
0.5 mM KCl, 0.5 mM NaCl, 0.1mM MgCl2, 0.2 mM CaCl2, 2.5% sucrose,pH 5.7

作者:北京林业大学陈金焕、戴逢斌

英文摘要

Salt stress is a major constraint for many crops and trees. A wild species of Goji named Lycium ruthenicum is an important economic halophyte in China and has an extremely high tolerance to salinity. L. ruthenicum grows in saline soil and is known as a potash-rich species.

However, its salt adaptation strategies and ion balance mechanism remains poorly understood. Potassium (K+) is one of the essential macronutrients for plant growth and development. In this study, a putative salt stress-responsive gene encoding a HAK (high-affinity K+)/KUP (K+ uptake)/KT (K+ transporter) transporter was cloned and designated as LrKUP8.

This gene belongs to the cluster II group of the KT/HAK/KUP family. The expression of LrKUP8 was strongly induced under high NaCl concentrations. The OE-LrKUP8 calli grew significantly better than the vector control calli under salt stress conditions. Further estimation by ion content and micro-electrode ion flux indicated a relative weaker K+ efflux in the OE-LrKUP8 calli than in the control.

Thus, a key gene involved in K+ uptake under salt condition was functionally characterized using a newly established L. ruthenicum callus transformation system. The importance of K+ regulation in L. ruthenicum under salt tolerance was highlighted.

中文摘要(谷歌机翻)

盐胁迫是许多农作物和树木的主要限制因素。枸杞的一种野生种,称为枸杞,是中国重要的经济盐生植物,对盐分的耐受性极高。ruthenicum生长在盐渍土壤中,被称为富含钾肥的物种。

然而,其盐适应策略和离子平衡机制仍然知之甚少。钾(K+)是植物生长和发育必不可少的大量营养素之一。在这项研究中,克隆了编码HAK(高亲和力K+)/ KUP(K+摄取)/ KT(K+转运蛋白)转运蛋白的假定盐胁迫响应基因,并将其命名为LrKUP8。

该基因属于KT / HAK / KUP家族的II类。在高NaCl浓度下强烈诱导LrKUP8的表达。在盐胁迫条件下,OE-LrKUP8愈伤组织的生长明显好于载体对照的愈伤组织。通过离子含量和微电极离子通量的进一步估计表明,与对照相比,OE-LrKUP8愈伤组织中的K+外排相对较弱。

因此,使用新建立的黑麦草愈伤组织转化系统对在盐条件下参与钾离子吸收的关键基因进行了功能鉴定。强调了在耐盐性条件下钌中的K+调节的重要性。

结果表明:LrKUP8抑制了愈伤组织中K+外排。不存在NaCl胁迫时,两种愈伤组织的K+外排速率较小,且无显著差异。经盐胁迫后,K+外排速率远高于无胁迫组;对照组(pBI121)的K+外排速率明显高于过表达组(OE-LrKUP8)。过表达组由于其更强的保K+能力,因而维持高的K+/Na+比。保持最优的K+/Na+比是植物耐盐性的关键特征之一,证明LrKUP8的保K+能力促进了黒枸杞在盐胁迫下的生长发育。

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